ISCB-Asia/SCCG 2012 Keynote Address
Photo Max Brouwers
The goal of our own work is to provide quantitative data on the energetics and kinetics of membrane protein assembly in vivo to serve as a basis both for a deeper understanding of the mechanism of membrane protein assembly, and to help improve topology- and structure-prediction methods.
The most important achievements include the discovery and experimental validation of the so-called “(-1,-3)-rule” (describes signal peptide cleavage sites; EJB 133:17, JMB 184:99) and the “positive inside” rule (describes membrane protein topology; EMBO J 5:3021, Nature 341:456, Cell 62:1135, Cell 77:401), the development of widely used prediction methods (e.g., TopPred, SignalP, TargetP, TMHMM; JMB 225:487, Prot.Engineer. 10:1, JMB 300:1005, JMB 305:567, JMB 327:735, JMB 340:783, PNAS 105:7177), the first proteome-wide theoretical and experimental studies of membrane protein topology in E. coli and S. cerevisiae (Protein Sci. 7:1029, Science 308:1321, JMB 35:489, PNAS 103:11142), the first quantitative analysis of the energetics of membrane protein assembly in vivo (Nature 433:377, Science 307:1427, Nature 450:1026, PNAS 106:11588), and recent theoretical and experimental studies of so-called dual-topology membrane proteins and their role in the evolution of membrane protein structure (Nature Struct.Mol.Biol. 13:112, Science 315:1282, Science 328:1698).
Altogether, the cumulated work has been cited ~46,000 times, h-index = 85 (Web of Science). Listed in the ISI Highly Cited database.
Prof. Heijne has recieved numerous awards, including the 2012 ISCB Senior Scientist Award.