Molecular characterization of NKR-p1 receptor in peripheral blood of pig

Banshi Sharma1, CVL

The aim of the work is to characterize the NKR-P1 receptor in the peripheral blood of pig. The strategy of reverse transcriptase for obtaining cDNA has been utilized. When first stand of cDNA synthesis occurs, subsequently PCR has been done. PCR is optimized with annealing temperature and extension time. It is obtained empirically. Total RNA was extracted from peripheral blood of pig as well as human. RT-PCR gives 573 bp PCR product in human. Similarly it gives 661 bp PCR product in pig.