FatiGO: a web tool for finding significant associations of Gene Ontology terms with groups of genes

Fatima Al-Shahrour1, Ramon Diaz-Uriarte2, Joaquin Dopazo
1falshahrour@cnio.es, Bioinformatics Unit, Spanish Natonal Cancer Center, CNIO.; 2rdiaz@cnio.es, Bioinformatics Unit, Spanish Natonal Cancer Center, CNIO.

We present a simple but powerful procedure to extract Gene Ontology terms that are significantly over or under-represented in sets of genes within the context of a genome-scale experiment (DNA microarray, proteomics, etc.). Said procedure has been implemented as a web application, FatiGO (http://fatigo.bioinfo.cnio.es), allowing for easy and interactive querying. FatiGO, which takes the multiple-testing nature of statistical contrast into account, currently includes GO associations for diverse organisms (human, mouse, fly, worm and yeast) and the TrEMBL/Swissprot GOAnnotations@EBI correspondences from the European Bioinformatics Institute.