| 2018 Bioscope Winter School | |
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Portugal - Lisbon - Caparica |
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| Hosted by: | BIOSCOPE GROUP |
| Venue: | he Faculty of Sciences and Technology (FCT NOVA), Campus of Caparica (Lisbon, portugal) |
| Dates: | Feb 26, 2018 through Mar 07, 2018 |
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Description |
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| We are organizing the Bioscope Winter School in February-March 2018, in Caparica (Lisbon, Portugal), as follows: 05th March - 07th March 2018 VIII Hands-on Course in Sample Preparation Using Nanoparticles for Proteomics In this course attendees learn how to make the synthesis of gold nanoparticles and about how to use them to reduce the complexity of complex proteomes such as serum or plasma. Module I - Theory Synthesis and characterization of nanoparticles Nanoparticle-based sample fractionation Electrophoresis Protein identification by Mass Spectrometry Nano-profiling Module II - Hands-on Sample clean up Protein quantification Nanoparticle synthesis characterization and applications: Protein fractionation using gold-nanoparticles (serum samples) Characterization of GNPs-protein complexes (UV-Vis, SDS-PAGE) In-gel and In-solution protein digestion (GNPs-protein complexes) Protein identification using MALDI-TOF/TOF MS Data analysis 26th - 28th February 2018 IX Hands-on Course in Ultrafast Sample Treatment for Proteomics In this course attendees learn how to do ultrafast shot gun proteomics and how to profile diseases or other type os samples using complex proteomes. Module I - Theory Practical aspects of sample collection storage and preservation for the proteome analysis Fundamentals of proteomics Protein fractionation: 1D-GE, 2D-GE, off-gel IEF Sample preparation: In-gel trypsin digestion Protein identification by Mass Spectrometry Module II - Hands-on Sample clean-up Protein separation: 2D gel electrophoresis In-gel trypsin digestion: accelerated method MALDI-TOF/TOF-MS and nLC-MS/MS data acquisition Data analysis: Protein identification |
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| Additional Information | |
| Event URL: | http://www.bioscopegroup.org/images/courses/2018%20BIOSCOPE%20Training%20Courses.pdf |
| ISCB Member Discount: | None |
| Contact Person: | 00351919404933 ([javascript protected email address]) |
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