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Monday, July 24, between 18:00 CEST and 19:00 CEST
Tuesday, July 25, between 18:00 CEST and 19:00 CEST
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Monday, July 24, between 08:00 CEST and 08:45 CEST
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Monday, July 24, at 19:00 CEST 		Session B Poster Set-up and Dismantle
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Tuesday, July 25, between 08:00 CEST and 08:45 CEST
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Tuesday, July 25, at 19:00 CEST
Wednesday, July 26, between 18:00 CEST and 19:00 CEST
Session C Poster Set-up and Dismantle
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Wednesday, July 26,between 08:00 CEST and 08:45 CEST
Session C Posters dismantle:
Wednesday, July 26, at 19:00 CEST
Virtual
Potential Complex RNA Pseudoknot Structures found in Internal Transcribed Spacer (ITS) rRNA Xylanase Producing Fungi obtained from Zea maize
Track: iRNA
  • Noel Unciano, Industrial Technology Development Institute - DOST, Philippines
  • Frank Heralde III, University of the Philippines Manila, College of Medicine, Philippines
  • Ursela Bigol, Industrial Technology Development Institute - DOST, Philippines


Presentation Overview: Show

Nature reagents from fungi of extreme diverse properties offer rich source of molecular foundry RNA reagents. The internal transcribed spacer (ITS) nuclear rRNA is now considered a long noncoding RNA with the recent discovery of SRP, RNase P, and RNase MRP RNAs in the ITS region of ectomycorrhizal fungi. This study explore molecular architectures, describes the RNA pseudoknot in ITS of local xylanase fungi from environmental samples with GenBank accession numbers MK459553-Mk459561.
VsFold5.23 predicted 4 domains RNA pseudoknot structures for: Aspergillus sp., Fusarium sp., and Neocosmospora sp.; while 7 domains for Scedosporium sp. Complex pseudoknot structures representing crossing-pseudoknot are visualized with RNAProbe prediction on chemical probing data with the IPknot++ webserver. Homologous ITS for clinical isolates were from the ISHAM (The International Society for Human and Animal Mycology) database for morphometric analysis. Structural distance between the secondary RNA structures and the ISHAM homolog was by computation recognition algorithms implemented in Crossalign.
The secondary structure of ITS rRNA differ from the clinical isolates and exhibited more negative minimal free energy (mfe). The presence of pseudoknot structures as well as potential CRISPR spacer site at single stranded RNA region could provide versatile tool for various applications of molecular diagnostics.