| EMBL Course: Advanced training with Oxford Nanopore Technologies | |
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Germany - 69117 - Meyerhofstraße 1 |
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| Hosted by: | The European Molecular Biology Laboratory |
| Venue: | EMBL Advanced Training Centre |
| Dates: | Jan 14, 2019 through Jan 18, 2019 |
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Description |
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| The goal of this course is to teach nanopore sequencing using the Oxford Nanopore Technologies (ONT) platform. We will start with an introduction into ONT technology and devices, with the goal of covering end to end workflows for the preparation and analysis of human and yeast samples using whole genome and barcoded cDNA sequencing approaches. This course will cover the wet lab preparation of libraries from genomic DNA and total RNA, with a focus on the critical steps and potential pitfalls and understanding what constitutes a ‘good’ sample for purpose of best results using the technology. The training includes an overview of the MinKNOW GUI for GridION and MinION devices. We then cover methods available for basecalling and analysis of samples for structural variants and differential gene expression, using both Oxford Nanopore and open source tools. Audience: This course is directed towards molecular biologists, bioinformaticians, scientists and technicians who want to apply Oxford Nanopore Sequencing technology in their labs. Knowledge of ONT sequencing technology is desirable but not essential. |
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| Additional Information | |
| Event URL: | https://www.embl.de/training/events/2019/NAN19-01/ |
| ISCB Member Discount: | None |
| Contact Person: | EMBL Events ([javascript protected email address]) |
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