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EMBL Course Hands-on course for sample preparation and data analysis in proteomics
Germany - BW - Heidelberg

Hosted by: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Dates: Dec 03, 2024 through Dec 06, 2024

The course will give an introduction to the theoretical aspects of the analysis of proteins/peptides by mass spectrometry. Participants will get hands-on experience in sample preparation for a quantitative comparison across samples and perform an in-solution digest using Single-Pot Solid-Phase-enhanced Sample Preparation (SP3) (Hughes et al., Molecular Systems Biology, 2014) and tandem mass tag labelling (TMT). The SP3 technology was developed at EMBL. It enables scientists with a proteomics sample preparation method from nearly any starting buffer conditions (e.g. Laemmli buffer).
Participants will measure their own produced samples using LC-MS/MS and will learn in a session focused on the bioinformatics analysis how the acquired data will be analysed using MSFragger and R. Best practices and Proteomics data management will be a focus of this course.
Additional Information
Event URL: https://www.embl.org/about/info/course-and-conference-office/events/sdp24-01/
ISCB Member Discount: None

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